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Norepinephrine Stimulation of Phosphoinositide Hydrolysis in Rat Cerebral Cortex Is Associated with the Alpha 1‐ Adrenoceptor
Author(s) -
Schoepp Darryle D.,
Knepper Sheila M.,
Rutledge Charles O.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06106.x
Subject(s) - prazosin , yohimbine , inositol phosphate , endocrinology , inositol , medicine , agonist , phenylephrine , alpha (finance) , stimulation , norepinephrine , chemistry , antagonist , adrenergic receptor , biology , receptor , dopamine , construct validity , nursing , blood pressure , patient satisfaction
Norepinephrine (NE) and the selective alpha‐adrenoceptor agonist phenylephrine (PE) both markedly stimulate the formation of [ 3 H]inositol phosphates in a concentration‐dependent manner upon incubation with [ 3 H]myo‐inositol. The selective alpha 2 agonist, clonidine, did not significantly alter [ 3 H]inositol phosphate formation, even at concentrations as high as 10 ‐3 M . The alpha, antagonist prazosin (IC 50 , 0.036 μ M ) was 300 times more potent than the alpha 2 antagonist yohimbine (IC 50 , 10.7 μ M ) as an inhibitor of NE (10 ‐4 M )‐stimulated phospha‐tidylinositol (PI) hydrolysis. These results indicate that the alpha 1 ‐, but not the alpha 2 ‐adrenoceptor subtype in rat brain is coupled to phosphoinositide hydrolysis.