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Photoaffinity Labeling of Different Benzodiazepine Receptors at Physiological Temperature
Author(s) -
Eichinger A.,
Sieghart W.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06103.x
Subject(s) - flunitrazepam , photoaffinity labeling , benzodiazepine , chemistry , receptor , cerebellum , gabaa receptor , ligand (biochemistry) , hippocampus , biochemistry , biophysics , neuroscience , biology
Irreversible labeling of benzodiazepine receptors in membranes from cerebellum or hippocampus was compared at 0°C or 37°C using [ 3 H]flunitrazepam as a photoaffinity ligand. [ 3 H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P 51 ) in cerebellum and at least two proteins (P 51 and P 55 ) in hippocampus at both temperatures. Differential inhibition at 37°C of irreversible [ 3 H]flunitrazepam binding to the individual proteins by several selective benzodiazepine receptor ligands supports the hypothesis that P 51 and P 55 are associated with different benzodiazepine receptors.