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Elevation of Intracellular Cyclic AMP and Stimulation of Adenylate Cyclase Activity by Vasoactive Intestinal Peptide and Glucaeon in the Retinal Pigment Epithelium
Author(s) -
Koh ShavWhey M.,
Chader Gerald J.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06072.x
Subject(s) - vasoactive intestinal peptide , adenylate kinase , glucagon , medicine , endocrinology , intracellular , cyclase , stimulation , neuropeptide , retinal pigment epithelium , biology , peptide hormone , chemistry , retinal , biochemistry , hormone , receptor
Both vasoactive intestinal peptide (VIP) and glucagon rapidly elevated cyclic AMP levels in embryonic chick retinal pigment epithelium (RPE), in culture as well as in freshly dissected tissue. In cultured cells, the half‐maximal activities of VIP and glucagon were 5 × 10 ‐8 M and 3 × 10 ‐8 M , respectively. After 3 min of reaction, VIP elevated intracellular cyclic AMP by 100‐fold; elevation with glucagon was up to 10‐fold. Both neuropeptides stimulated adenylate cyclase activity in RPE membranes. Glucagon showed a half‐maximal activity of 1 × 10 ‐8 M. VIP remained more effective than glucagon in stimulating adenylate cyclase activity, but the doseresponse curve was shifted to a higher concentration range when compared to that of the VIP‐elevated intracellular cyclic AMP.