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Extraction and Partial Characterization of Enkephalin‐Like Peptides from Splanchnic Nerve
Author(s) -
Hexum Terry D.,
Russett Linda R.,
Barren Barbara A.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb05417.x
Subject(s) - splanchnic nerves , adrenal medulla , chemistry , splanchnic , medulla , enkephalin , endocrinology , medicine , chromatography , catecholamine , biochemistry , receptor , stimulation , biology , opioid , blood flow
A method is described for the extraction of enkephalin‐like peptides from peripheral nerve using chloroform and acidic methanol to facilitate a differential extraction of peptides and lipid. Porcine splanchnic nerve contains enkephalin‐like peptides in low amounts compared to porcine adrenal medulla and striatum. Gel filtration chromatography reveals the presence of enkephalinlike peptides in both processed and cryptic forms. This is the first reported isolation and partial characterization of these peptides in splanchnic nerve. The presence of these peptides in this nerve provides support for the contention that the splanchnic nerve can modulate catecholamine release from the adrenal medulla through an effect on opiate receptors located on chromaffin cells.