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Single Doses of Acrylamide Reduce Retrograde Transport Velocity
Author(s) -
Miller Matthew S.,
Spencer Peter S.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb05400.x
Subject(s) - acrylamide , anatomy , chemistry , neuroscience , medicine , biology , physics , nuclear magnetic resonance , copolymer , polymer
Single doses of acrylamide (0–1.3 mmol/kg) produced a dose‐dependent decrease in the transport of 125 I‐tetanus toxin to the perikarya of sensory neurons in dorsal root ganglia and motor neurons in ventral spinal cord. Acrylamide was a more potent inhibitor of retrograde transport in sensory axons than in motor axons. Substantially greater doses of N,N ′‐methylene‐bis‐acryl‐amide, a reportedly non‐neurotoxic analog of acrylamide, were required to alter the axonal transport of 125 I‐tetanus toxin. Velocity of retrograde transport was assessed by determining the position of the leading edge of transported 125 I‐tetanus toxin at times following single doses of acrylamide. Acrylamide reduced the velocity of 125 I‐tetanus toxin transport in a dose‐dependent manner by up to 75%. No change in neuronal uptake of 125 I‐tet‐anus toxin was detected. It is concluded that single doses of acrylamide produce profound alterations in retrograde transport which precede the appearance of structural changes in affected nerve fibers.