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Hormonal Regulation of Adipose S‐100 Protein Release
Author(s) -
Suzuki Fujiko,
Kato Kanefusa,
Nakajima Takashi
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb05391.x
Subject(s) - lipolysis , epinephrine , medicine , endocrinology , propranolol , adipose tissue , incubation , phentolamine , chemistry , hormone , adrenergic , norepinephrine , biology , biochemistry , receptor , dopamine
Abstract: The release of S‐100 protein from epididymal fat pads was enhanced by epinephrine in vitro , and about 50% of S‐100 protein in the tissue was released into the medium after 2‐h incubation at 37°C with 10 μ M epinephrine. Similar results were obtained with the incubation of isolated adipocytes. The S‐100 protein release was also enhanced by isoproterenol, norepinephrine, ACTH, and dibutyryl cyclic AMP, which all increase the lipolysis by increasing cyclic AMP levels in the tissue. Propranolol, a β‐adrenergic blocker, could block the increase of S‐100 protein release by catecholamines, indicating that the release was mediated by the β‐adrenergic effect of catecholamines. However propranolol had no suppressive effect on the enhancement of S‐100 protein release by ACTH or dibutyryl cyclic AMP. Insulin had an inhibitory effect on the epinephrine‐enhanced S‐100 protein release. Epinephrine or ACTH could not stimulate the S‐100 protein release in the absence of Ca 2+ , whereas the epinephrine‐enhanced glycerol release was not affected under the same conditions. The increase in S‐100 protein release was induced by only a pretreatment of the tissue with epinephrine. However, the lipolysis in the tissue was not enhanced by the pretreatment alone. These results indicate that the release of S‐100 protein from adipocytes is regulated by the hormones that have been known to control the lipolysis with a manner slightly different from that of lipolysis.