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Effect of Intravenous Administration of D‐Lysergic Acid Diethylamide on Initiation of Protein Synthesis in a Cell‐Free System Derived from Brain
Author(s) -
Cosgrove James W.,
Brown Ian R.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb02803.x
Subject(s) - methionine , protein biosynthesis , lysergic acid diethylamide , cell , chemistry , amino acid , in vitro , pharmacology , biochemistry , biology , receptor , serotonin
An initiating cell‐free protein synthesis system derived from brain was utilized to demonstrate that the intravenous injection of D‐lysergic acid diethylamide (LSD) to rabbits resulted in a lesion at the initiation stage of brain protein synthesis. Three inhibitors of initiation, edeine, poly(I), and aurintricarboxylic acid were used to demonstrate a reduction in initiation‐dependent amino acid incorporation in the brain cell‐free system. One hour after LSD injection, there was also a measurable decrease in the formation of 40S and 80S initiation complexes in vitro , using either [ 35 S]methionine or [ 35 S]Met‐tRNA f . Analysis of the methionine pool size after LSD administration indicated there was no change in methionine levels. Analysis of the formation of initiation complexes in the brain cell‐free protein synthesis system prepared 6 h after LSD administration indicated that there was a return to control levels at this time. The effects of LSD on steps in the initiation process are thus reversible.