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Effects of Polyamines on the Uptake of Neurotransmitters by Rat Brain Synaptosomes
Author(s) -
Law CheLeung,
Wong Patrick C. L.,
Fong WangFun
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb02761.x
Subject(s) - putrescine , spermidine , cadaverine , spermine , glycine , biochemistry , choline , chemistry , polyamine , synaptosome , biology , in vitro , amino acid , enzyme
The influence of putrescine, spermidine, spermine, and some aliphatic α,ω‐diamines on the uptake of neurotransmitters by rat forebrain synaptosomes was investigated. Choline uptake was most effectively inhibited by spermine (IC 50 = 0.22 m M ), less so by spermidine (IC 50 = 4.0 m M ), but not by putrescine (IC 50 > 100 m M ). At 10 m M, 1,3‐diaminopropane, cadaverine, and 1,8‐diaminooctane all inhibited choline uptake by 50% or more. Spermine and spermidine inhibited the uptake of dopamine with IC 50 values of 2.7 and 2.2 m M , respectively. Putrescine was only slightly inhibitory (IC 50 = 17.3 m M ) and the other diamines were inactive. The uptake of γ‐aminobutyrate (GABA) was only slightly inhibited (15–40%) by the polyamines at 10 m M . With the exception of inhibition of glycine uptake by 1,8‐diaminooctane (60%) and of glutamate uptake by cadaverine (35%) none of the polyamines, tested at 10 m M , affected the uptake of adenosine, glutamate, and glycine significantly. A possible modulatory role for polyamines in synaptic transmission through interaction by negatively charged groups of the synaptic membrane with the polycationic compounds is discussed.