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Genetic Study of Cationic ATPase Activities and Audiogenic Seizure Susceptibility in Recombinant Inbred and Congenic Strains of Mice
Author(s) -
Palayoor Sanjeewani T.,
Seyfried Thomas N.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb02710.x
Subject(s) - congenic , inbred strain , recombinant dna , biology , genetics , gene
Total, Mg 2+ , and Na + , K + ATPase activities were studied in fresh brain homogenates of the audiogenic seizure (AGS)‐resistant C57BL/6J (B6) and AGS‐susceptible DBA/2J (D2) inbred strains and in 13 B6 × D2 (BXD) recombinant inbred (RI) strains. These activities were also studied in the D2.B6‐ Ias b congenic mice, that are similar genetically to D2 mice, except for the Ias b gene which inhibits the spread of AGS activity. The total and Mg 2+ ATPase activities of the brainstem were significantly lower in the D2 than in the B6 mice at 21 days of age. No differences were found between these strains for Na + , K + ATPase activity. The total, Mg 2+ , and Na + , K + ATPase activities in the B6 brainstem did not change noticeably from 21 to 80 days of age. In the D2 brainstem, however, the Mg 2+ activity increased with age, and the Na + , K + ATPase activity decreased from 30 to 80 days of age. No genetic associations could be found between AGS susceptibility and total or Mg 2+ ATPase activities in the D2.B6‐ Ias b mice or among the 13 BXD RI strains. Hence, differences in genetic background, rather than differences in AGS susceptibility, can account for the lower ATPase activities in 21‐day‐old D2 mice. Further, the Mg 2+ and Na + , K + ATPase activities appear to be regulated by more than one gene. This study emphasizes the utility of RI and congenic strains for testing the biochemical basis of AGS susceptibility in mice.