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Respiration in Primary Cultured Cerebellar Granule Neurons and Cerebral Cortical Neurons
Author(s) -
Jameson Nancy,
Olson James,
Nguyen Hung,
Holtzman David
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb02701.x
Subject(s) - respiration , cerebellum , biology , cerebral cortex , cortical neurons , granule (geology) , neuroscience , stimulation , oligomycin , medicine , anatomy , biochemistry , paleontology , atpase , enzyme
Respiration was measured polarographically in primary cultures enriched with cerebellar granule neurons or cerebral cortical neurons. The basal respiratory rate, measured on the sixth day after culturing, was 12.00 natom equiv. O/mg protein/min for the cortical neurons and 12.70 natom equiv. O/mg protein/min for the granule neurons. Maximal stimulation by 2,4‐dinitrophenol produced a 20–40% increase over the basal rate for both neuronal types. Oligomycin inhibited neuronal basal respiration by 45%. These respiratory rates in neurons from primary culture are markedly lower than those measured in astrocytes grown under similar conditions.