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Evidence for the Presence of Lectins with Mannose Specificity in the Rat Cerebellum
Author(s) -
Zanetta J. P.,
Reeber A.,
Dontenwill M.,
Vincendon G.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb02683.x
Subject(s) - mannose , lectin , horseradish peroxidase , biochemistry , glycoprotein , cerebellum , glucosamine , chemistry , glycan , in vitro , glycoconjugate , biology , microbiology and biotechnology , enzyme , neuroscience
Two different methods were set up to detect the possible presence of lectin‐like molecules with a specificity for mannose‐rich glycans in the rat cerebellum. The first, affinity histochemistry, involved the isolation of a particular class of glycoproteins from the cerebella of 11‐day‐old rats followed by the formation of covalent complexes with horseradish peroxidase and then incubation with cerebellar slices. The second used in vitro interactions between [ 3 H]leucine‐labeled proteins, kept in solution, with insolubilized [ 14 C]glucosamine‐labeled glyco proteins. The results of both methods are compatible with the presence of lectin‐like activities inhibited by high mannose concentrations, but not other sugars. However, the binding sites preferred by these molecules seem to be more than a single mannose residue.