Premium
β‐Adrenergic Binding Sites in Fetal Rat Brain
Author(s) -
Bruinink A.,
Lichtensteiger W.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb00937.x
Subject(s) - forebrain , endocrinology , dihydroalprenolol , dissociation constant , medicine , adrenergic agent , binding site , fetus , ontogeny , adrenergic , chemistry , biology , biochemistry , central nervous system , receptor , pregnancy , genetics , partial agonist , antagonist
The ontogeny of β‐adrenergic binding sites was studied in forebrain homogenates from male and female rats. Specific [ 3 H]dihydroalprenolol binding was defined by the difference between binding in the presence and in the absence of 330 n M (+)oxprenolol. Significant binding was detected at prenatal stages. The dissociation constants ( K D of [ 3 H]dihydroalprenolol binding were similar at gestational day (GD) 19 3/4 and postnatal day (PN) 31. Binding was first detected in forebrain at GD 15 3/4 . The amount of binding sites increased until PN 31, when adult values were reached. No sex differences could be detected at any of the stages tested (GD 19 3/4 ‐PN 31).