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Specific Receptor‐Mediated Inhibition of Cyclic AMP Synthesis by Dopamine in a Neuroblastoma × Brain Hybrid Cell Line NCB‐20
Author(s) -
BerryKravis Elizabeth,
Freedman Stephen B.,
Dawson Glyn
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb00917.x
Subject(s) - yohimbine , medicine , sulpiride , endocrinology , dopamine , dopamine receptor , apomorphine , cyclase , chemistry , adenylate kinase , dopamine receptor d1 , agonist , receptor , stimulation , biology , antagonist , dopaminergic
Dopamine and dopamine receptor agonists were found to inhibit adenylate cyclase activity dose‐de‐β ndently in a neuroblastoma × Chinese hamster brain explant hybrid cell line NCB‐20. Apomorphine (with an IC 50 value of 10 n M ) was the most effective inhibitor, followed by 2‐amino‐6,7‐dihydroxy‐1,2,3,4‐tetrahydro‐naphthaline (ADTN), dopamine, and N ‐dipropyldopa‐mine. The inhibition was potently reversed by sulpiride, butaclamol, and flupenthixol in a stereospecific manner, but was unaffected by yohimbine, except at high concentrations. Clonidine also inhibited adenylate cyclase activity in these cells and this was reversed by the α 2 ‐adrenoreceptor antagonist yohimbine, but not by sulpiride. [ d ‐Ala 2 , d ‐Leu 5 ]Enkephalin inhibited adenylate cyclase activity in NCB‐20 cells at nanomolar concentrations; this was reversed by naloxone. All three inhibitory neurotransmitters were able to reverse the stimulation of cyclic AMP synthesis by serotonin or prostaglandin E 1 The dopamine receptor that modulates cyclic AMP synthesis in NCB‐20 cells is pharmacologically quite distinct from a high‐affinity spiperone binding site identified in these cells, but shows the pharmacologic specificity of the D 2 receptor previously described in mammalian brain.