Premium
Production and Characterization of Monoclonal Antibodies to Peripheral and Central Nervous System Myelin
Author(s) -
Miller Sheldon L.,
Pleasure David,
Herlyn Meenhard,
Atkinson Barbara,
Ernst Carolyn,
Tachovsky Thomas G.,
Baird Lynn
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb00914.x
Subject(s) - myelin , monoclonal antibody , glycoprotein , antibody , biology , myelin basic protein , myelin associated glycoprotein , central nervous system , clone (java method) , peripheral nervous system , microbiology and biotechnology , immunology , biochemistry , endocrinology , dna
Monoclonal antibodies against P0, myelin basic protein, or myelin‐associated glycoprotein were generated by fusing mouse myeloma cells with spleen cells from BALB/c mice immunized with central and peripheral nervous system myelin proteins. The antibodies secreted were either IgG, IgM, or IgA. Clone C6B5 (iso‐type IgM) secreted antibody(ies) that bound to both myelin basic protein and myelin‐associated glycoprotein, although binding of antibody to myelin basic protein as detected by the immunoblot technique appeared to be much less than to the myelin‐associated glycoprotein. Antibodies were characterized in solid‐phase radioimmunoassay for their species cross‐reaction, and histologically for the specificity of binding to myelin in central and peripheral nervous system tissues. These monoclonal reagents should prove valuable in studying CSF and myelin‐producing cells, since in both cases the concentration of myelin proteins is low.