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Increased Binding of [ 3 H]GABA to Striatal Membranes Following Ischemia
Author(s) -
Francis Andrew,
Pulsinelli William
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb13598.x
Subject(s) - striatum , ischemia , gabaergic , membrane , binding site , forebrain , chemistry , medicine , receptor , endocrinology , biochemistry , biophysics , biology , central nervous system , dopamine
Sodium‐independent binding of [ 3 H]γ‐aminobutyric acid ([ 3 H]GABA) to membranes prepared from ischemic‐damaged rat striatum was studied by kinetic and time‐course analysis. Three days after 40 min of ischemia, [ 3 H]GABA binding increased fourfold over control values. Scatchard analysis of the binding showed that ischemia significantly increased the affinity ( K D ) and the total number of binding sites (B max ) for the high‐affinity GABA receptor. These results support the conclusion that transient forebrain ischemia damages striatal GABAergic neurons.