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High Levels of Brain Dolichols in Neuronal Ceroid‐Lipofuscinosis and Senescence
Author(s) -
Kin N. M. K. Ng Ying,
Palo J.,
Haltia M.,
Wolfe L. S.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb13592.x
Subject(s) - dolichol , neuronal ceroid lipofuscinosis , cerebral cortex , lipofuscin , biology , senescence , cortex (anatomy) , biochemistry , niemann–pick disease , human brain , medicine , endocrinology , enzyme , biosynthesis , microbiology and biotechnology , neuroscience , cholesterol , gene
Dolichols as unesterified alcohols were identified as significant components of lipid extracts from storage cytosomes isolated post‐mortem from the brains of patients with the infantile, late infantile, and juvenile types of neuronal ceroid‐lipofuscinosis (NCL). Very small amounts of dolichols were present in the corresponding subcellular fractions of non‐NCL brains. The nuclear fraction from NCL cerebral cortex contained the highest dolichol content expressed per milligram protein or lipid, whereas the crude mitochondrial fraction was the richest in normal brain. Highly significant elevations of dolichol levels were found in human cerebral cortex of patients with NCL and Alzheimer's disease compared with age‐matched controls, but the levels were normal in Pick's disease. In human non‐NCL cerebral cortex, dolichols increased from 16 μg/g at age 5 to over 200 at age 81. Rat cerebral cortex showed a similar progressive increase in dolichol content with age. The high dolichol values in NCL, Alzheimer's disease, and senescence appears to be related to the increase of lipofuscin in brain. This is the first time a uniform biochemical abnormality has been found in all childhood forms of NCL, but the enzyme defect is still unidentified. It may lie on pathways where dolichols and retinyl compounds are recycled in Golgi membranes and derived organelles during the biosynthesis of glycoproteins.