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Proteins of the Extracellular Fluid of Mouse Brain: Extraction and Partial Characterization
Author(s) -
Hofstein Raphael,
Hesse Gary,
Shashoua Victor E.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb13589.x
Subject(s) - extracellular , extracellular fluid , cytoplasm , biochemistry , extraction (chemistry) , chemistry , enzyme , mammalian brain , chromatography , biology , molecular mass , neuroscience
An extraction procedure for the isolation of proteins from the brain extracellular fluid (ECF) was developed and applied to studies of the ECF components of mouse brain. Perfused intact brains were incubated in an isotonic medium for periods of up to 2 h at 0°C to allow the release of ECF into the medium without disruption of the integrity of the tissue. The validity of the extraction procedure was established by (a) the fact that the total yield of ECF proteins was constant per unit weight of brain tissue, (b) the absence of tyrosine hydroxylase, an enzyme marker of the cytoplasmic fraction, from the extracts, and (c) the distinctive features of the one‐ and two‐dimensional gel electrophoretic patterns of ECF proteins as compared with those of the cytoplasmic and membrane fractions. The results indicate that the extracellular fluid of mouse brain contains a mixture of proteins with a wide distribution of molecular weights (10,000–100,000 daltons) at a concentration level of about 0.3%.