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Acetylcholine Synthesis and Accumulation in the CNS of Drosophila Larvae: Analysis of shibire ts , a Mutant with a Temperature‐Sensitive Block in Synaptic Transmission
Author(s) -
Wu ChunFang,
Berneking James M.,
Barker David L.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb13581.x
Subject(s) - acetylcholine , choline , tetrodotoxin , neurotransmitter , neurotransmission , intracellular , mutant , chemistry , cholinergic , biophysics , biochemistry , biology , endocrinology , receptor , gene
A radiochemical method is applied to the study of neurotransmitter metabolism in Drosophila . The larval CNS is a favorable system for analyzing acetylcholine (ACh) metabolism, since the pool of [ 3 H]ACh rapidly reaches a steady state with a high ratio of intracellular [ 3 H]ACh to [ 3 H]choline. A temperature‐sensitive paralytic mutant, shibire ts , shows reduced [ 3 H]ACh accumulation at the restrictive temperature. This reduction is not the result of decreased synthesis of [ 3 H]ACh, but rather an abnormally rapid rate of release, which is not prevented by blocking tetrodotoxin‐sensitive nerve activity.