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Stimulation of Free Fatty Acid and Diacylglycerol Accumulation in Cerebrum and Cerebellum During Bicuculline‐Induced Status Epilepticus. Effect of Pretreatment with α‐Methyl‐ p ‐Tyrosine and p ‐Chlorophenylalanine
Author(s) -
Turco Elena B. Rodriguez de,
Liberti Susana Morelli,
Bazan Nicolas G.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb12679.x
Subject(s) - bicuculline , arachidonic acid , cerebrum , medicine , endocrinology , chemistry , phosphatidylinositol , docosahexaenoic acid , cerebellum , biochemistry , fatty acid , polyunsaturated fatty acid , biology , gabaa receptor , central nervous system , receptor , kinase , enzyme
The pool size and composition of free fatty acids (FFA) and diglycerides (DG) from the cerebrum and cerebellum of rats undergoing bicuculline‐induced seizures were studied. A fourfold increase in cerebral FFA occurred 3–4 min after bicuculline injection; arachidonic and stearic acids were the principal fatty acids accumulated. Cerebellar FFA also increased, but to a lesser extent. An increased production of arachidonic acid took place in the cerebrum as a function of time after bicuculline injection. Other fatty acids produced were oleic, palmitic, and docosahexaenoic acids. A twofold increase in cerebral arachidonic acid was seen at the time of the first generalized tonic‐clonic convulsion. However, a 13‐ to 17‐fold increase in arachidonic acid was seen approximately 5–6 min after bicuculline injection. The rise in other FFA was much smaller. Stearoyl‐ and arachidonoyl‐DG were also accumulated. The drug α‐methyl‐ p ‐tyrosine was found to (a) potentiate the bicuculline‐stimulated release of cerebellar FFA, and (b) inhibit by 70% the production of stearoyl‐ and arachidonoyl‐DG in the cerebrum and cerebellum. Basal production of FFA was stimulated by p ‐chlorophenylalanine, but the drug had no effect on the bicuculline‐induced changes. Hydrolysis of phospholipids enriched in stearoyl‐arachidonoyl groups, such as phosphatidylinositol of excitable membranes, may be stimulated during seizures.