Premium
Effects of In Vivo Hypoxia on Acetylcholine Synthesis by Rat Brain Synaptosomes
Author(s) -
Booth R. F. G.,
Harvey S. A. K.,
Clark J. B.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb12659.x
Subject(s) - veratridine , acetylcholine , synaptosome , hypoxia (environmental) , in vivo , chemistry , endocrinology , medicine , in vitro , biochemistry , biology , oxygen , sodium , sodium channel , microbiology and biotechnology , organic chemistry
Synaptosomes from normoxic and hypoxic rats were incubated aerobically in the presence and absence of veratridine. In the absence of veratridine, no significant difference was observed between the two types of preparation regarding either ATP/ADP ratio or 14 CO 2 or [ 14 C]acetylcholine synthesis from D‐[U‐ 14 C]glucose. However, in the presence of veratridine, significant reductions in the output of 14 CO 2 and [ 14 C]acetylcholine by synaptosomes from hypoxic rats were apparent. It was concluded that irreversible metabolic lesions occur at the synapse as a result of hypoxia, which are apparent only when the metabolism of the preparation is accelerated to a level comparable with the maximal rate occurring in vivo. The presence of such lesions is further evidenced by the significant reductions in ATP/ADP ratio, 14 CO 2 output, and [ 14 C]acetylcholine synthesis that occur in synaptosomes from hypoxic rats made anoxic in vitro and permitted to recover. Such decreases are not seen when synaptosomes from normoxic rats are similarly treated.