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On the Phospholipid Metabolism of Glial Cell Primary Cultures.
Author(s) -
Witter Brigitte,
Gunawan Johannes,
Debuch Hildegard
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb12653.x
Subject(s) - plasmalogen , phosphatidylethanolamine , phospholipid , phosphatidylcholine , ethanolamine , alkyl , chemistry , biochemistry , acylation , metabolism , incubation , choline , substrate (aquarium) , biology , organic chemistry , ecology , membrane , catalysis
Primary cultures were prepared from newborn rat brain. After 16‐18 days, they consisted mainly of mature and immature astrocytes and oligodendrocytes, as judged by immunohistochemistry. To study the metabolism of ethanolamine glycerophospholipids, the cells were incubated with 1‐[1‐ 3 H]alkyl‐ sn ‐glycero‐3‐phosphoethanolamine (1‐alkyl‐GPE), for 1–20 h. Five main products were formed: 1‐alkyl‐2‐acyl‐GPE; 1‐alkyl‐2‐acyksn‐glycero‐3‐phosphocholine (1‐alkyl‐2‐acyl‐GPC); 1‐alkenyl‐2‐acyl‐GPE (ethanolamine plasmalogen); 1‐alkenyl‐2‐acyl‐GPC (choline plasmalogen); and 1‐alkyl‐glycerol. Acylation of the substrate was the main reaction during the first 3 h of incubation, whereas desaturation to plasmaiogen reached a maximum after 12 h. Greater amounts of radioactivity were observed in the phosphatidylcholine fraction after longer incubation times. Only small amounts of choline plasmalogen were observed. The phosphatidylethanolamine fraction consisted of 26.5% diacyl‐, 27.5% alkyl‐acyl‐, and 46.0% alkenyl‐acyl‐ compounds, whereas the corresponding data for the phosphatidylcholine fraction were 78.5, 16.4, and 5.1%, respectively, after 20 h of incubation. Hydrolysis of the substrate to 1‐alkyl‐glycerol was a minor reaction.