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In Vitro Studies on the Effect of β‐Carbolines on the Activities of Acetylcholinesterase and Choline Acetyltransferase and on the Muscarinic Receptor Binding of the Rat Brain
Author(s) -
Skup Małgorzata,
OderfeldNowak Barbara,
Rommelspacher Hans
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb11814.x
Subject(s) - acetylcholinesterase , choline acetyltransferase , muscarinic acetylcholine receptor , in vitro , chemistry , aché , choline , neuroscience , receptor , central nervous system , endocrinology , medicine , pharmacology , biology , biochemistry , enzyme
Acetylcholinesterase (acetylcholine acetylhy‐drolase, EC 3.1.1.7) activity and muscarinic receptor binding of homogenates from several brain structures were inhibited by β‐carbolines. The inhibition was of the noncompetitive type in the case of the enzyme and of the mixed type in the case of the receptor binding. This effect was most strongly manifested by pyridoindoles(harmane, norharmane), i.e., carbolines containing an aromatic C ring than by the corresponding piperidoindoles (tetrahy‐droharmane, tetrahydronorharmane), i.e., those with a reduced C ring. The activity of choline acetyltransferase (acetyl‐CoAxholine O ‐acetyltransferase, EC 2.3.1.6) was not altered. These data are further evidence of the interactions between indoleamine derivatives and the cholinergic system. The results are discussed in terms of their possible biological significance.