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Distribution and Properties of Thiol S ‐Methyltransferase in Rat Brain
Author(s) -
Hiemke Christoph,
Ghraf Rüdiger
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb11325.x
Subject(s) - dithiothreitol , thiol , medulla oblongata , methyltransferase , chemistry , hippocampus , microsome , methylation , biochemistry , biophysics , biology , endocrinology , enzyme , central nervous system , dna
Microsomal thiol S ‐methyltransferase (TMT) of rat brain catalysed the methylation of dithiothreitol ( K m = 84 μ M ) and other thiol compounds using S ‐adenosylmethionine as methyl donor ( K m = 3.7 μ M ). With increasing polarity of thiol substrates there was a decrease in the maximal velocities of reaction and an increase in the apparent K m values. TMT was found to be unevenly distributed amongst various brain regions, with highest activities in the medulla oblongata and the hippocampus.