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High‐Affinity, Sodium‐Dependent Γ‐Aminobutyric Acid Uptake by Slices of Rat Ovary
Author(s) -
Erdó Sándor L.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb11322.x
Subject(s) - nipecotic acid , ouabain , ovary , sodium , chemistry , alanine , aminobutyric acid , biochemistry , biophysics , medicine , endocrinology , biology , amino acid , receptor , neurotransmitter , organic chemistry
The high concentration of γ‐aminobutyric acid (GABA) recently demonstrated in rat ovary prompted us to examine the capacity of ovarian slices to take up [ 3 H]‐GABA. Active uptake, dependent on temperature and sodium concentration, was observed and a kinetic constant (K m ) of 1.0 μ M found for the uptake process. Ouabain (100 μ M ) reduced the rate of accumulation of [ 3 H]GABA. Uptake was inhibited only partially by 100 μ M d, l ‐nipecotic acid, but more strongly by 100 μ M β‐alanine. These results suggest that the uptake system in ovary possesses properties similar to those of high‐affinity GABA transport systems in the brain.