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Reductions of Γ‐Aminobutyric Acid and Glutamate Uptake and (Na + + K + )‐ATPase Activity in Brain Slices and Synaptosomes by Arachidonic Acid
Author(s) -
Chan Pak Hoo,
Kerlan Randi,
Fishman Robert A.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb11284.x
Subject(s) - arachidonic acid , synaptosome , chemistry , biochemistry , glutamate receptor , atpase , sodium , aminobutyric acid , membrane , fatty acid , enzyme , receptor , organic chemistry
Arachidonic acid, a major polyunsaturated fatty acid of membrane phospholipids in the CNS, reduced the high‐affinity uptake of glutamate and γ‐aminobutyric acid (GABA) in both rat brain cortical slices and synaptosomes. α‐Aminoisobutyric acid uptake was not affected. Intrasynaptosomal sodium was increased concomitant with decreased (Na + + K + )‐ATPase activity in synaptosomal membranes. The reduction of GABA uptake in synaptosomes could be partially reversed by α‐tocopherol. The inhibition of membrane‐bound (Na + +K + )‐ATPase by arachidonic acid was not due to a simple detergent‐like action on membranes, since sodium dodecyl sulfate stimulated the sodium pump activity in synaptosomes. These data indicate that arachidonic acid selectively modifies membrane stability and integrity associated with reductions of GABA and glutamate uptake and of (Na + + K + )‐ATPase activity.