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In Vitro Protein Synthesis in the Goldfish Retinotectal Pathway During Regeneration: Evidence for Specific Axonal Proteins of Retinal Origin in the Optic Nerve
Author(s) -
Quitschke Wolfgang,
Schechter Nisson
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb09063.x
Subject(s) - optic nerve , retina , regeneration (biology) , retinal , ganglion , giant retinal ganglion cells , in vitro , microbiology and biotechnology , biology , retinal ganglion cell , chemistry , anatomy , neuroscience , biochemistry
Four proteins with molecular weights of 58,000 can be separated as a linear array by two‐dimensional gel electrophoresis. They are highly concentrated in the goldfish optic nerve and are designated as ON 1 , ON 2 , ON 3 , and ON 4 . Proteins ON 1 and ON 2 are undetectable in the optic nerve after disconnection and their concentration is gradually restored during regeneration. In vitro incubations of retinas, optic nerves, or tecta in the presence of [ 35 S]methionine indicate that proteins ON 1 and ON 2 are of retinal origin. The labeling rate of these proteins in the retina increases fourfold after optic nerve crush whereas the overall labeling rate in the retina remains largely constant. Their synthesis cannot be detected in tissues de void of retinal ganglion cells. This is consistent with the view that ON 1 and ON 2 are synthesized by retinal ganglion cells and are consequently of neuronal origin in the optic nerve. In contrast, similar experiments indicate that ON 3 and ON 4 are of nonneuronal origin. They are synthesized in the optic nerve in the absence of retinal ganglion cells.