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Presence of Somatostatin, Enkephalins, and Substance P‐Like Peptides in Cultured Neurons from Embryonic Chick Cerebral Hemispheres
Author(s) -
Louis J. C.,
Rougeot C.,
Bepoldin O.,
Vulliez B.,
Mandel P.,
Dray F.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb09036.x
Subject(s) - somatostatin , leu enkephalin , enkephalin , endocrinology , medicine , substance p , neuropeptide , radioimmunoassay , chemistry , peptide hormone , dopaminergic , hormone , biology , biochemistry , dopamine , receptor , opioid
The presence of peptides in pure cultures of neurons from 8‐day‐old chick embryo cerebral hemispheres has been investigated by means of specific radioimmunoassays and chromatographic purification. Somatostatin, Met‐enkephalin, Leu‐enkephalin, and substance P immunoreactive substances have been detected in 8‐day‐old cultures grown in serum‐free culture medium. The peptides were present in the cellular extracts, as well as in the culture medium extracts. β‐Endorphin, thyroliberin, luteinizing hormone‐releasing hormone, and ACTH could not be detected. The largest amount was accounted by somatostatin (48 ± 2 ng/mg protein). Some 60% of the somatostatin‐immunoreactive material was found in the culture medium. Met‐enkephalin, Leuenkephalin, and substance P were present at lower concentrations: 1.61 ± 0.27, 0.24 ± 0.02, and 0.14 ± 0.005 ng/mg protein, respectively. The identities of somatostatin‐ and enkephalin‐immunoreactive materials were confirmed by high pressure liquid chromatography. The findings suggest that cultured neurons that express dopaminergic and GABAergic properties contain peptides similar, if not identical, to somatostatin, Met‐enkephalin, Leu‐enkephalin, and substance P.