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The Nerve Growth Factor Receptor on PC 12 Cells: Interconversion Between Two Forms with Different Binding Properties
Author(s) -
Block Timothy,
Bothwell Mark
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08139.x
Subject(s) - receptor , cytoskeleton , nerve growth factor , 3t3 cells , microbiology and biotechnology , cell surface receptor , trypsin , biophysics , membrane , cell , cell membrane , biology , chemistry , cell culture , biochemistry , enzyme , transfection , genetics
PC 12 cells possess two classes of nerve growth factor (NGF) receptors on their surfaces which can be distinguished by kinetic criteria. The majority class binds and releases 125 I‐NGF at a relatively rapid rate and has been called fast . The second class of receptors has been called slow because of relatively slower rates of binding and release of 125 I‐NGF, and also may be distinguished from fast receptors by their cytoskeletal association and resistance to trypsin. PC 12 cell plasma membranes were prepared and shown to have only the fast class of receptors. These membranes were fused to receptorless 3T3 cells with polyethylene glycol. The resultant fused cells were shown to possess NGF receptors, essentially all of which behave like slow receptors. Immunofluorescence microscopy was used to monitor the introduction of PC 12 cell membrane and NGF receptorsinto 3T3 cells. Results obtained with C10‐2, a monoclonal antibody specific for a major PC12 cell‐surface antigen, show that up to 90% of 3T3 cells receive PC 12 membrane and that the PC 12 membrane becomes integrally incorporated into the 3T3 cell plasma membrane. It is suggested that an association of receptors with cytoskeleton may be involved in the conversion of fast to slow receptor behavior, and that the differing proportion of fast and slow NGF receptors in PC 12 and 3T3 cells reflects the differing cytoskeletal organization of these cells.