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Local Cerebral Glucose Utilization in Two Models of B 12 Deficiency
Author(s) -
Hakim Antoine M.,
Cooper Bernard A.,
Rosenblatt David S.,
Pappius Hanna M.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08107.x
Subject(s) - vitamin b12 , endocrinology , medicine , cerebral cortex , chemistry , homocysteine , methionine , biochemistry , amino acid
Local cerebral glucose utilization (LCGU), as measured by the 2‐deoxy‐D‐[l‐ 14 C]glucose technique, reflects local cerebral functional activity. In an effort to elucidate mechanisms of the encephalopathy associated with deficiency of vitamin B 12 , LCGU was determined in two recently described models of effective B 12 deficiency: exposure of rats to subanesthetic doses of nitrous oxide (N 2 O) and/or administration of 1‐amino‐cyclopentane‐1‐carboxylic acid (cycloleucine). Our results show that exposure of adult rats to N 2 O depresses LCGU selectively in cortical, auditory, and limbic structures, in association with a depression in whole‐brain activities of the vitamin B 12 ‐dependent methyltetrahydrofolate‐homocysteine methyl‐trans‐ferase (EC 2.1.1.13, methionine synthetase). Cycloleucine has no discernible effect on LCGU in the adult rat and does not change the cerebral activity of methionine synthetase.