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Inhibition of Calcium Uptake, Sodium Uptake, and Catecholamine Secretion by Methoxyverapamil (D600) in Primary Cultures of Adrenal Medulla Cells
Author(s) -
Corcoran James J.,
Kirshner Norman
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08099.x
Subject(s) - veratridine , adrenal medulla , chemistry , endocrinology , tetrodotoxin , medicine , gallopamil , catecholamine , calcium , nicotine , chromaffin cell , voltage dependent calcium channel , sodium , sodium channel , biology , nifedipine , organic chemistry
The calcium‐entry antagonist D600 (methoxyverapamil) inhibited nicotine‐ and veratridine‐induced ‐ 45 Ca‐ 4 uptake, 22 Na + uptake, and catecholamine secretion in primary cultures of bovine adrenal medulla cells. Inhibition of nicotine‐induced effects occurred at D600 concentrations approximately 3‐10‐fold lower than those needed to produce similar inhibition of veratridine‐induced effects. Inhibition of the veratridine‐induced effects was competitive, but inhibition of the nicotine‐induced effects was not competitive. These re‐sults suggest that D600, in addition to blocking “slow” Ca‐ channels and tetrodotoxin‐sensitive Na + channels also blocks nicotine transmission, possibly either by noncompetitively inhibiting the interaction of nicotine with the receptor binding site or by blockade of the receptor‐associated ion conductance channel.