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Characterization and Location of Met 5 ‐Enkephalin‐Arg 6 ‐Phe 7 Stored in Various Rat Brain Regions
Author(s) -
Yang H.Y. T.,
Panula P.,
Tang J.,
Costa E.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08080.x
Subject(s) - enkephalin , antiserum , chemistry , met enkephalin , radioimmunoassay , neuropeptide , peptide , proenkephalin , biochemistry , leu enkephalin , endocrinology , biology , antibody , receptor , immunology , opioid
A specific antiserum against met 5 ‐enkepha‐lin‐arg 6 ‐phe 7 was raised and used to study the distribution and characterization of met 5 ‐enkephalin‐arg 6 ‐phe 7 ‐like immunoreactive material in rat brains by radioimmunoassay and immunohistochemical procedures. The antiserum appears to be directed to the COOH‐terminus of the peptide, as it fails to cross‐react with met 5 ‐enkeph‐alin, met 3 ‐enkephalin‐arg 6 , met 5 ‐enkephalin‐arg 6 ‐arg 7 , met 6 ‐enkephalin‐lys 6 , and leu‐enkephalin. However, it cross‐reacts with phe‐met‐arg‐phe by about 10% and with phe‐met‐arg‐phe‐NH 2 to an insignificant degree. The highest content of met 5 ‐enkephalin‐arg 6 ‐phe 7 was found in the striatum, which contains a dense network of immunoreactive varicose fibers and terminals, as well as immunoreac tive cell bodies. The met 5 ‐enkephalin‐arg 6 ‐phe 7 in striatum can be released in a Ca 2+ ‐dependent manner by a depolarizing concentration of KC1, raising the possibility of a neu‐roregulatory role for met 5 ‐enkephalin‐arg 6 ‐phe 7 . Characterization of the immunoreactive material by gel filtration and high pressure liquid chromatography revealed the presence of multiple forms of immunoreactive material in some brain regions.