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Purification and Characterization of the Heat‐Stable Factors Essential for the Conversion of Lignoceric Acid to Cerebronic Acid and Glutamic Acid: Identification of N ‐Acetyl‐ l ‐Aspartic Acid
Author(s) -
Shigematsu Hidenari,
Okamura Nobuyuki,
Shimeno Hiroshi,
Kishimoto Yasuo,
Kan Lousing,
Fenselau Catherine
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08052.x
Subject(s) - glutamic acid , biochemistry , aspartic acid , chemistry , amino acid
The conversion of lignoceric acid to cerebronic acid, ceramides, cerebrosides, and glutamic acid is catalyzed by a rat brain particulate preparation. The heat‐stable factor, prepared from calf cerebellum, together with the heat‐labile factor, a pyridine nucleotide, and Mg 2+ are essential to all of these metabolic pathways. Our previous work showed that the heat‐stable factor is composed of at least two components, HSF‐1 and HSF‐2, and identified HSF‐2 as d ‐glucose‐6‐phosphate. In the current investigation, HSF‐1 was further purified and found to be N ‐acetyl‐ l ‐aspartic acid. In addition, it was discovered that a third component, HSF‐3, is also required for heat‐stable factor activity. A reconstituted system composed of N ‐acetylaspartic acid, glucose‐6‐phosphate, and HSF‐3 fully replaced the heat‐stable factor essential for the conversion of lignoceric acid to cerebronic acid and glutamic acid. The reconstituted heat‐stable factor did not show the initial time lag always observed with the crude heat‐stable factor.