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Solid‐Phase Radioimmunoassay for Substance P
Author(s) -
Oblin Andre,
Zivkovic Branimir
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08047.x
Subject(s) - radioimmunoassay , substance p , chromatography , immunoassay , chemistry , antibody , neuropeptide , biochemistry , receptor , biology , immunology
The solid‐phase immunoassay for quantification of substance P has been developed. The assay is based on the repartition of anti‐substance P antibodies between the insoluble phase‐immobilized substance P and the free peptide. The immobilized substance P‐antibody complex is then quantified with 125 I‐protein A. The method allowed detection of 10 pg of substance P. The values of substance P concentration obtained by the present method in different regions of the rat brain were comparable to those obtained by standard radioimmunoassay with 125 I‐tyr‐8‐substance P as tracer. The described solid‐phase radioimmunoassay is a simple, sensitive, and reliable technique for quantification of substance P‐like immunoreactivity in biological samples.