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A Highly Sensitive Enzyme Immunoassay for Mouse β Nerve Growth Factor
Author(s) -
Furukawa Shoei,
Kamo Isao,
Furukawa Yoshiko,
Akazawa Saeko,
Satoyoshi Eijiro,
Itoh Koji,
Hayashi Kyozo
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08040.x
Subject(s) - immunoassay , nerve growth factor , enzyme , chemistry , biology , biochemistry , antibody , immunology , receptor
A sensitive two‐site enzyme immunoassay system for mouse β nerve growth factor (NGF) was developed, based on the sandwiching of the antigen between anti‐mouse β NGF antibody IgG coated to a polystyrene tube and anti‐mouse β NGF antibody Fab'‐linked β‐ d ‐galactosidase (β‐ d ‐galactoside hydrolase, EC 3.2.1.23). This method has the following advantages: (a) the procedures are simple and rapid compared to bioassay or two‐site radioimmunoassay; (b) antibody Fab'‐β‐ d ‐galactosidase complex is more stable than 125 I‐labeled antibody; (c) purified β NGF is detectable at a concentration as low as 10 pg/ml. Our enzyme immunoassay was used to examine the levels of NGF in some tissues of mice. The submaxillary gland contained a high concentration of NGF. However, other tissues, such as the heart, brain, and skeletal muscle, and serum did not contain detectable NGF. These results support recent findings by other investigators that NGF was not found in the organs/tissues other than the submaxillary gland of mice.