Premium
Identification and Determination of 1‐Methylimidazole‐4‐Acetic Acid in Human Cerebrospinal Fluid by Gas Chromatography‐Mass Spectrometry
Author(s) -
Swahn CarlGunnar,
Sedvall Göran
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb08034.x
Subject(s) - acetic acid , chemistry , chromatography , cerebrospinal fluid , gas chromatography–mass spectrometry , mass spectrometry , acetic anhydride , gas chromatography , detection limit , ethyl acetate , biochemistry , medicine , catalysis
A method for the quantification of 1‐methylimidazole‐4‐acetic acid in human CSF was developed. Methylimidazole‐acetic acid was identified and quantitated in CSF. The method involves concentration of the compound on a cation exchanger, extraction of the methyl ester with ethyl acetate, and preparation of a heptafluorobutyryl derivate of the methyl ester, which is finally purified by chromatography on silica gel and quantitated by gas chromatography‐mass spectrometry with the deuterated analogue as internal standard. The coefficient of variation at 1 ng/ml was 13%. The limit of sensitivity was about 0.2 ng/ml. The concentration of methylimidazole‐acetic acid in lumbar CSF from healthy volunteers was below 1 ng/ml. Ventricular CSF contained higher concentrations than lumbar fluid. The existence of a rostrocaudal concentration gradient was established. There was a correlation between the concentration of methylimidazole‐acetic acid and tele‐methylhistamine in CSF. The concentration of methylimidazole‐acetic acid in lumbar CSF from schizophrenic patients, patients with subarachnoidal haemorrhage, or patients with rheumatic disease was in the range of that in healthy volunteers.