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Effect of Oral Administration of Tri‐ o ‐cresyl Phosphate on In Vitro Phosphorylation of Membrane and Cytosolic Proteins from Chicken Brain
Author(s) -
Patton Suzanne E.,
O'Callaghan James P.,
Miller Diane B.,
AbouDonia Mohamed B.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04826.x
Subject(s) - phosphorylation , cytosol , in vitro , membrane protein , biochemistry , phosphate , polyacrylamide gel electrophoresis , protein phosphorylation , gel electrophoresis , endogeny , chemistry , sodium dodecyl sulfate , membrane , biology , protein kinase a , enzyme
The effects of a single oral dose of 750 mg/kg tri‐ o ‐cresyl phosphate (TOCP) on the endogenous phosphorylation of specific brain proteins were assessed in male adult chickens following the development of delayed neurotoxicity. Phosphorylation of crude synaptosomal (P 2 ) membrane and synaptosomal cytosolic proteins was assayed in vitro by using [γ‐ 32 P]ATP as phosphate donor. Following resolution of brain proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis, specific protein phosphorylation was detected by autoradiography and quantified by microdensitometry. TOCP administration enhanced the phosphorylation of both cytosolic (M r 65,000 and 55,000) and membrane (20,000) proteins by as much as 146% and 200%, respectively.