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Phosphoinositide Metabolism in the Retina: Localization to Horizontal Cells and Regulation by Light and Divalent Cations
Author(s) -
Anderson Robert E.,
Maude Maureen B.,
Kelleher Paula A.,
Rayborn Mary E.,
Hollyfield Joe G.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04806.x
Subject(s) - inositol , retina , phosphatidylinositol , xenopus , biophysics , biology , divalent , biochemistry , microbiology and biotechnology , cell type , metabolism , cell , chemistry , neuroscience , receptor , signal transduction , organic chemistry , gene
Isolated retinas from Xenopus laevis incorporated greater amounts of [ 3 H]inositol and 32 P i into phosphoinositides when incubated in light than did control retinas incubated in the dark. Inositol was primarily incorporated into phosphatidylinositol (83–86%), while phosphate labeled the polyphosphoinositides (72–79%). The incorporation of radioactive glycerol, serine, choline, or ethanolarnine into retinal lipids was unaffected by light. Following incubation with [ 3 H]inositol, the cell type involved in the light response was identified by light and electron microscope autoradiography to be the horizontal cell. These results are consistent with a classic phosphatidylinositol effect in the retina. An interesting feature of this response is that the stimulus (light) is received in the photoreceptor cell and the effect is manifest in the horizontal cell.