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Adenosine A1 Receptors Are Associated with Cerebellar Granule Cells
Author(s) -
Wojcik W. J.,
Neff N. H.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04805.x
Subject(s) - medicine , adenosine , endocrinology , cerebellum , granule cell , cyclase , adenylate kinase , purkinje cell , biology , receptor , granule (geology) , adenosine a1 receptor , adenosine receptor , central nervous system , stimulation , agonist , paleontology , dentate gyrus
The cerebellum of mouse appears to have only the adenosine A1 receptor, which decreases adenylate cyclase activity, and not the A2 receptor, which increases adenylate cyclase activity. The adenosine analog N 6 ‐(l‐phenylisopropyl)adenosine (PIA), stimulates the Al receptor in a membrane preparation and decreases basal adenylate cyclase activity by 40%. The EC 50 for PIA is approximately 50 n M . To associate the A1 receptor with a cerebellar cell type, three different neurological mutant mouse strains were studied: staggerer (Purkinje and granule cell defect), nervous (Purkinje cell defect), and weaver (granule cell defect). PIA was unable to effect a maximal decrease in adenylate cyclase activity of membranes prepared from cerebella of the staggerer and weaver mice in comparison with the respective littermate control mice. In contrast, membranes from nervous mice and their littermates showed similar PIA dose‐response curves. Moreover, the diminished PIA response observed in the weaver cerebellum, when compared with the control littermate, was not detected in the striatum. This suggests no overall brain defect in the adenosine A1 receptors coupled to adenylate cyclase of the weaver mouse. We conclude that a loss of granule cells coincides with an attenuated response to PIA, implying that the A1 receptors are associated with the granule cells of the cerebellum.