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Specific Binding of [ 3 H]Rauwolscine to α 2 ‐Adrenoceptors in Rat Cerebral Cortex: Comparison Between Crude and Synaptosomal Plasma Membranes
Author(s) -
Diop Laurent,
Dausse JeanPierre,
Meyer Philippe
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04798.x
Subject(s) - rauwolscine , membrane , sodium , chemistry , cerebral cortex , synaptosome , receptor , antagonist , biochemistry , medicine , biophysics , endocrinology , biology , organic chemistry , yohimbine
[ 3 H]Rauwolscine, a specific, potent, radiolabelled α 2 ‐antagonist, binds to distinct high‐ and low‐affinity α 2 ‐adrenoceptors in crude membrane preparations of the rat cerebral cortex. The concentration of high‐affinity α 2 ‐adrenoceptors was increased by addition of sodium ions or guanylnucleotides. In synaptosomal plasma membrane preparations, only the low‐affinity component was found. Neither sodium or guanylnucleotides caused any increase in the concentration of these low‐affinity receptors for [ 3 H]rauwolscine.