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Presynaptic Distribution of the Cholinergic‐Specific Antigen Chol‐1 and 5′‐Nucleotidase in Rat Brain, as Determined by Complement‐Mediated Release of Neurotransmitters
Author(s) -
Richardson Peter J.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04789.x
Subject(s) - choline acetyltransferase , biochemistry , choline , chemistry , cholinergic , endocrinology , cerebrum , 5' nucleotidase , medicine , biology , adenosine , central nervous system
Nerve terminals prepared from rat cortex and hippocampus were loaded with seven radioactive putative neurotransmitters (serotonin, noradrenaline, dopamine, γ‐aminobutyric acid, aspartate, glutamate, and taurine). The release of these transmitters, choline acetyltransferase, 3,4‐dihydroxyphenylalanine decarboxylase, enolase, and lactate dehydrogenase was monitored during complement‐mediated lysis. Three antisera were used: anti‐5′‐nucleotidase, anti‐Chol‐1, and anti‐rat cerebrum. Anti‐5′‐nucleotidase serum did not cause the release of any labelled transmitter or of any of the enzymes studied. Anti‐Choi‐1 serum released choline acetyltransferase and small amounts of enolase and lactate dehydrogenase. Antirat cerebrum caused the release of all seven transmitters, choline acetyltransferase, and small amounts of the other three enzymes. It was concluded that 5′‐nucleotidase was not present on any of the terminals studied, and that Chol‐1 is only present on cholinergic terminals.