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In Vivo Release from Cerebral Cortex of [ 14 C]Glutamate Synthesized from [U‐ 14 C]Glutamine
Author(s) -
Thanki C. M.,
Sugden D.,
Thomas A. J.,
Bradford H. F.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04785.x
Subject(s) - glutamine , glutamate receptor , glutaminase , stimulation , cerebral cortex , cortex (anatomy) , biology , medicine , endocrinology , in vivo , glutamic acid , depolarization , biophysics , amino acid , chemistry , biochemistry , neuroscience , receptor , microbiology and biotechnology
Awake, unrestrained, and behaviourally normal animals with superfusion cannulae implanted over the sensorimotor cortex were used in a study of the capacity of infused [U‐ 14 C]glutamine for labelling glutamate and other amino acids released by depolarising stimuli. A spontaneous background release of [ 14 C]glutamate was detected. This was increased by tityustoxin (1 μM). The specific radioactivity of glutamate increased eightfold during the evoked‐release period. [ 14 C]Aspartate was also detected and showed increased release, but not increased specific labelling, in response to depolarisation. Evoked γ‐aminobutyric acid (GABA) release occurred but only small amounts of [ 14 C]GABA were detected. Glutamine showed increased rates of uptake to the sensorimotor cortex during stimulation periods, suggesting an accelerated breakdown via glutaminase.