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Cellular Localization of Gangliosides in the Developing Mouse Cerebellum: Analysis Using the Weaver Mutant
Author(s) -
Seyfried T. N.,
Miyazawa N.,
Yu R. K.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04767.x
Subject(s) - synaptogenesis , ganglioside , cerebellum , granule cell , biology , granule (geology) , microbiology and biotechnology , neurite , purkinje cell , mutant , biochemistry , dentate gyrus , neuroscience , central nervous system , in vitro , paleontology , gene
The distribution of gangliosides was studied in the weaver ( wv/wv ) mutant mouse, where the vast majority of postmitotic granule cell neurons die prior to their differentiation. The wv mutation also shows a dosage effect, as granule cell migration is slowed or retarded in the + /wv heterozygotes. By correlating changes in ganglioside composition with the well‐documented histological events that occur during cerebellar development in the normal (+/+), heterozygous ( +/wv ), and weaver ( wv/ wv ) mutant mice, information was obtained on the cellular localization and function of gangliosides. Ganglioside G M1 may be enriched in granule cell growth cones and play an important role in neurite outgrowth. A striking accumulation of G M1 , which may result from altered metabolism, occurred in the adult wvlwv mice. G D3 was heavily concentrated in undifferentiated granule cells, but was rapidly displaced by the more complex gangliosides during differentiation. G D1a became enriched in granule cells during formation of synaptic and dendritic membranes, whereas G T1a appeared enriched in Purkinje cell synaptic spines. A possible fucose‐containing ganglioside was quantitated only in the wvlwv mice. Ganglioside G T1b became enriched in granule cells during synaptogenesis, whereas G Q1b became enriched in these cells after synaptogenesis. The concentrations of G T1b and especially G Q1b increased continuously with age. Our results provide further evidence for a differential cellular enrichment of gangliosides in the mouse cerebellum and also suggest that certain gangliosides may be differentially distributed within the membranes of these cells at various stages of development.