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An Improved Method for the Purification of “Light” Noradrenaline Vesicles from Rat Vas Deferens: Some Biochemical Characteristics
Author(s) -
Willems M. F.,
Potter W. P.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb04764.x
Subject(s) - isopycnic , differential centrifugation , vesicle , vas deferens , centrifugation , free nerve ending , sucrose gradient , sucrose , chemistry , chromatography , biology , biochemistry , endocrinology , enzyme , membrane
“Light” noradrenaline storage vesicles from nerve endings have been isolated by differential centrifugation and differential gradient centrifugation. They have been further purified by isopycnic sucrose/D 2 O centrifugation. By using these centrifugation techniques, we obtained an isopycnic gradient fraction in which noradrenaline was enriched about 41 times versus a total homogenate. This factor could be raised to 61 by using seminal ducts of castrated rats. Comparison of the distribution patterns in sucrose/D 2 O isopycnic gradients indicated that light noradrenaline vesicles of nerve endings contain Mg 2+ ‐stimulated ATPase and ATP, but that only a minor part of the dopamine β‐hydroxylase can be associated with these vesicles.