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Effects of Temperature on Arachidonic Acid‐Induced Cellular Edema and Membrane Perturbation in Rat Brain Cortical Slices
Author(s) -
Chan Pak Hoo,
Fishman Robert A.,
Chen Sylvia,
Chew Susan
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00863.x
Subject(s) - arachidonic acid , phosphatidylinositol , biochemistry , intracellular , chemistry , incubation , lipoxygenase , enzyme , signal transduction
The effects of temperature on arachidonic acid‐induced cellular edema in the first cortical brain slices of rats were studied. Incubation of the cortical slice in arachidonic acid at 37°C induced cellular swelling, and increased intracellular Na + and lactic acid contents concomitant with decreased intracellular K + When the incubation temperature was reduced these changes were reduced in severity. The uptake of [ 3 H]arachidonic acid in cortical slices was temperature‐dependent. The incorporation of [ 3 H]arachidonic acid into various lipid fractions was further studied by HPLC. The majority of [ 3 H]arachidonic acid was incorporated into triacylglycerol and phosphatidylinositol (PI), but the incorporation of [ 3 H]arachidonic acid into PI was temperature‐dependent, unlike that into other phospholipids and neutrolipids. Further, cortical (Na + + K + )‐ATPase activity was inhibited whereas its subunit K + ‐activated p ‐nitrophenyl‐phosphatase was activated by arachidonic acid at various incubation temperatures. The effects of arachidonic acid on these enzymes is similar to that of thimerosal, a lipid removal agent. These data suggest that both temperature and arachidonic acid play an important role in the development of cellular edema associated with membrane perturbation and inactivation of (Na + + K + )‐ATPase activity.