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Use of Cholate/Sodium Chloride for Solubilisation of Brain D 2 Dopamine Receptors
Author(s) -
Hall Jean M.,
Frankham Patricia A.,
Strange Philip G.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00859.x
Subject(s) - receptor , spiperone , chemistry , centrifugation , sodium , dopamine receptor , chromatography , biochemistry , ligand (biochemistry) , sodium cholate , organic chemistry
The use of cholate (in the presence of sodium chloride) is described for solubilising brain D 2 dopamine receptors. The method described gives in high yield a preparation of solubilised D 2 receptors which when assayed by [ 3 H]spiperone binding show little interference from nonspecific and nonstereospecific binding sites. Characterisation by ultrafiltration, electron microscopy, gel filtration, and sucrose density gradient centrifugation has been achieved, showing the receptors to be truly solubilised. Pharmacological characterisation using [ 3 H]spiperone binding suggested the presence of serotonergic S 2 receptors in addition to D 2 receptors. The pharmacological properties of the solubilised D 2 receptors show a good correlation with those of membrane‐bound receptors, although ligand binding affinities are lower in the solubilised preparation.