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Pyruvate Carboxylase Activity in Primary Cultures of Astrocytes and Neurons
Author(s) -
Yu A. C. H.,
Drejer J.,
Hertz L.,
Schousboe A.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00849.x
Subject(s) - pyruvate carboxylase , astrocyte , enzyme , cerebral cortex , enzyme assay , biology , biochemistry , specific activity , endocrinology , medicine , chemistry , central nervous system
The activity of the pyruvate carboxylase was determined in brains of newborn and adult mice as well as primary cultures of astrocytes, of cerebral cortex neurons, and of cerebellar granule cells. The activity was found to be 0.25 ± 0.14, 1.24 ± 0.07, and 1.75 ± 0.13 nmol min −1 mg −1 protein in, respectively, neonatal brain, adult brain, and astrocytes. Neither of the two types of neurons showed any detectable enzyme activity (i.e., < 0.05 nmol min −1 mg −1 ). It is therefore concluded that pyruvate carboxylase is an astrocytic enzyme.