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Is Lysolecithin an In Vivo Constituent of Chromaffin Granules?
Author(s) -
Frischenschlager Inge,
Schmidt Wolfgang,
Winkler Hans
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00848.x
Subject(s) - adrenal medulla , in vivo , guinea pig , medulla , catecholamine , lysophosphatidylcholine , adrenal cortex , chromaffin cell , chemistry , biology , biochemistry , endocrinology , phosphatidylcholine , phospholipid , membrane , microbiology and biotechnology
It was recently claimed that lysolecithin (lysophosphatidylcholine) in chromaffin granules is a postmortem artefact. We have, therefore, determined catecholamine/lysolecithin ratios in adrenal tissues and isolated chromaffin granules. In rat adrenals and bovine medulla the ratios in both tissues and granules were similar. This indicates that even in rapidly frozen rat adrenal glands, sufficient lysolecithin is present in the total tissue to account for its presence in isolated organelles. Owing to the high cortex/medulla ratios such studies cannot be performed with guinea pig or rabbit adrenals. However, isolated chromaffin granules from guinea pig, in contrast to a previous study, do contain lysolecithin. We conclude that lysolecithin is an in vivo constituent of chromaffin granules of all species so far investigated.