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The Rate of Utilization of Glucose Via Hexosemonophosphate Shunt in Brain
Author(s) -
Gaitonde M. K.,
Evison E.,
Evans G. M.
Publication year - 1983
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1983.tb00819.x
Subject(s) - medicine , endocrinology , chemistry , pentose phosphate pathway , carbohydrate metabolism , glucose 6 phosphate , metabolism , biochemistry , biology , glycolysis , enzyme
The concentration of 6‐phosphogluconate in the brain increased from 0–24 nmol/g in the controls to 1430 and 1506 nmol/g in rats treated with 50 mg of 6‐aminonicotinamide/kg of body weight. A dose‐dependent increase in the concentrations of glucose and glucose 6 phosphate as well as of 6‐phosphogluconate was found in the brains of 6‐aminonicotinamide‐treated rats. The biochemical changes and symptoms of neurological disorder in 6‐aminonicotinamide‐treated rats were not due to hypothermia. The rate of utilization of glucose via the hexosemonophosphate shunt was determined by isolation of gluconate from 6‐phosphogluconate and measurement of its [ 14 C]content at short time intervals after injection of [U‐ 14 C]glucose into 6‐aminonicotinamide‐treated rats; it was 16.5 nmol of glucose utilized/min per g of brain, and represented approximately 2.3% of the overall utilization of glucose in the brain. A highly significant correlation was observed between the concentration of 6‐phosphogluconate and the concentration of glucose 6‐phosphate and free glucose. The validity of this correlation was supported by the results of previous investigations involving several other treatments.