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Differential Expression of Type A and Type B Monoamine Oxidase of Mouse Astrocytes in Primary Cultures
Author(s) -
Yu P. H.,
Hertz L.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb12598.x
Subject(s) - monoamine oxidase , astrocyte , in vitro , primary culture , endocrinology , monoamine oxidase a , monoamine oxidase b , biology , medicine , monoamine neurotransmitter , chemistry , microbiology and biotechnology , biochemistry , enzyme , serotonin , central nervous system , receptor
Type A and type B monoamine oxidase (MAO) activities were determined in mouse brain and in primary cultures of mouse astrocytes. Thirty‐one‐day‐old astrocyte cultures exhibited predominantly type A MAO activity. In cultures of the same age, treated with 0.25 mM dibutyryl cyclic AMP under the same culturing conditions, 30% type B MAO was expressed, although dibutyryl cyclic AMP up to 1 mM does not affect MAO activity in vitro. The specific activity of type B MAO increased significantly in older cultures, while type A MAO changed only slightly.