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Neurochemical Studies of the Mesolimbic Dopaminergic Pathway: [ 3 H]Spiperone Labels Two Binding Sites in Homogenates of the Nucleus Accumbens of Rat Brain
Author(s) -
Beart P. M.,
McDonald Danielle
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb12591.x
Subject(s) - nucleus accumbens , spiperone , chemistry , dopaminergic , apomorphine , striatum , medicine , endocrinology , neurochemical , sulpiride , dissociation constant , dopamine , dopamine receptor , biochemistry , receptor , biology
The binding of [ 3 H]spiperone to membranes of the nucleus accumbens of the rat brain was studied in vitro and found to be of high affinity, rapid, saturable, reversible and stereospecific. Dissociation and saturation experiments indicated the presence of two specific binding sites with apparent dissociation constants of 70 p M and >1 n M . Specific binding with 25 p M [ 3 H]spiperone represented >90% of total binding and was displaced by dopaminergic agonists, neuroleptic drugs and ergot derivatives. The rank order of potency for the ergot derivatives was bromocryptine > pergolide > lergo‐trile, and that for D‐2 antagonists was domperidone > sulpiride > molindone > metoclopramide. Noradrenergic, histaminergic and serotonergic components of the binding were not detected. [ 3 H]Spiperone binds to high‐affinity sites in homogenates of nucleus accumbens, which are likely to be D‐2 receptors.